Histological Structure of Regenerate Tail in Lizard (Mabouya multifasciata Kuhl) After Treatment Using Shark Cartilage Ointment
Keywords:
histology, lizard, shark cartilageAbstract
Lizards are grouped into the order Sauria which has the characteristics of a scaly body, smooth, long tongue, long tail, and four legs. Lizards (Mabouya multifasciata Kuhl) will sever its tail as a self-protection mechanism. After the autotomy event, the tail regeneration process will be followed to replace the severed tail. Shark cartilage contains bioactive compounds that are beneficial for humans, including for the wound healing process. Shark cartilage extract has been used medicinally to treat pain and swelling, treat scars, and for the prevention of arthritis. The lizards were grouped into four groups before being treated using shark cartilage extract ointment. The formulation of shark cartilage extract ointment used in this study consisted of four types, that is control, 5%, 10%, and 15%. The purpose of this study was to determine the effect of shark cartilage extract ointment on the histological structure of the autotomy-set lizard regenerate tail. The method used is the paraffin method with hematoxylin-eosin staining. The results of this study showed that the 5% formulation of the ointment made the blastema layer thicker and perivertebral fat tissue more abundant.
References
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Alibardi L. 2017. FGFs Treatment on Amputated Lizard Limbs Stimulate the Regeneration of Long Bones, Opening New Avenues for Limb Regeneration in Amniotes: A Morphological Study. Journal of Functional Morphology and Kinesiology 2 (25): 1-17.
Albardi L and VB Meyer-Rochow. 2021. Regeneration in Reptiles Generally and the New Zealand Tuatara in Particular as a Model to Analyse Organ Regrowth in Amniotes: A Review. Journal of Developmental Biology 9 (36): 1-21.
Al-Saadi HM, K Lun-Pang, SI Nirwana, K Yong-Chin. 2019. Multifaceted Protective Role of Glucosamine against Osteoarthritis: Review of Its Molecular Mechanisms. Scientia Pharmaceutica 87 (34): 1-18.
Apriyanto P, AH Yanti, TR Setyawati. 2015. Keragaman Jenis Kadal Sub Ordo Sauria pada Tiga Tipe Hutan di Kecamatan Sungai Ambawang. Protobiont 4 (1): 108-114.
Avery RA. 1970. Utilization of caudal fat by hibernating common lizards, Lacerta vivipara. Comp. Biochem. Physiol 37: 119-121.
Bargahi A and A. Rabbani-Chadegani A. 2008. Angiogenic inhibitor protein fractions derived from shark cartilage. Journal Biochemical Society 28 (1): 15–21.
Borba FKSL, EVL Costa, VAB Polli, DS Coelho, M Maraschin, PF Dias and RA Nogueira. 2017. Pro-angiogenic Activity Assay of Chondroitin Sulfate and Glucosamine Sulfate on Vascular Network of Mouse and of Chick Embryo Chorioallantoic Membrane. Journal of Glycobiology 6 (3): 1-6.
Chang C, P Wu, RE Baker, PK Maini, L Alibardi, and CM Chuong. 2009. Reptile scale paradigm: Evo-Devo, pattern formation, and regeneration. The International Journal of Developmental Biology 53: 813-826.
Dean MN and AP Summers. 2006. Mineralized Cartilage in the skeleton of chondrichthyan fishes. Zoology 109 (2): 164-168.
Delorme SL, IM Lungu and MK Vickaryous. 2012. Scar-Free Wound Healing and Regeneration Following Tail Loss in the Leopard Gecko, Eublepharis macularius. The Anatomical Record 295 (10):1575–1595.
Ferreti P, F Zhang, LS Ruis, and JDW Clarke. 2001. FGF signalling and blastema growth during amphibian tail regeneration. International Journal Development Biology (45): 127-128.
Fisher RE, LA Geiger, LK Stroik, ED Hutchins, RM George, DF Denardo, K Kusumi, J Alan Rawls and J Wilson-Rawls. 2012. A Histological Comparison of the Original and Regenerated Tail in the Green Anole, Anolis carolinensis. The Anatomical Record 295 (10):1609–1619.
Gilbert RWD, MK Vickaryou,s and AM Viloria-Petit. 2013. Characterization of TGFb Signaling During Tail Regeneration in the Leopard Gecko (Eublepharis macularius). Developmental Dynamics 242:886–896.
Greer AE and DG Broadley. 2000. Six Characters of Systematic Importance In The Scincid Lizard Genus Mabuya. Hamadryad 25 (1): 1-12.
Hartomo BT, FG Firdaus. 2014. Pemanfaatan Biomaterial Kitosan Dalam Bidang Bedah Mulut. Jurnal Kedokteran Gigi Universitas Baiturrahmah 6 (1): 63-70.
Hutchins ED, Wilson-Rawls, and Kusumi. 2016. Innovations in Molecular Mechanisms and Tissue Engineering, Stem Cell Biology and Regenerative Medicine. Springer International Publishing Switzerland. Arizona State University, Phoenix. The USA. P:23-35.
Inayah N, NP Soesilo and R Pratiwi. 2017. Effect of Tokay Gecko (Gekko gecko LINNAEUS, 1758) Saliva on Angiogenesis During Wound Healing Phase of Autotomized Tail in Common Sun Skink (Eutropis multifasciata KUHL, 1820. Jurnal Biologi Indonesia 13 (2): 253-260.
Jacyniak K, RP McDonald, MK Vickaryous. 2017. Tail regeneration and other phenomena of wound healing and tissue restoration in lizards. Journal of Experimental Biology 220 (16): 2858-2869.
Jagnandan K, AP Rus,sell and TE Higham. 2014. Tail autotomy and subsequent regeneration alter the mechanics of locomotion in lizards. The Journal of Experimental Biology (217):3891-3897.
Jerosch J. 2011. Effects of Glucosamine and Chondroitin Sulfate on Cartilage Metabolism in OA: Outlook on Other Nutrient Partners Especially Omega-3 Fatty Acids. International Journal of Rheumatology. Page: 1-18.
Kent GC. 1987. Comparative Anatomy of the Vertebrates. Sixth edition. Times Mirror/ Mosby College Publishing. UThe nited States of America. p: 192.
Landreneau EB. 2011. Scales and Scale-Like Structures. Dissertation. College Station: Texas A&M University
Londono R, Wenzhong, Wang, RS Tuan, and TP Lozito. 2017. cartilage and Muscle cell Fate and Origins during lizard Tail regeneration. Frontiers in Bioengineering and Biotechnology 5 (70): 1-9.
Lopez-senra E, P Casal-Beiroa, M López-Álvarez, J Serra, P González, J Valcarcel, JA Vázquez, EF Burguera, FJ Blanco, and J Magalhães. 2020. Impact of Prevalence Ratios of Chondroitin Sulfate (CS)- 4 and -6 Isomers Derived from Marine Sources in Cell Proliferation and Chondrogenic Differentiation Processes. Marine Drugs 18 (94): 1-13.
Lozito TP and RS Tuan. 2017. Lizard Tail Regeneration As An Instructive Model of Enhanced Healing Capabilities In An Adult Amniote. Connective Tissue Research 58 (2): 145–154.
Luthfi MJ. 2002. Kalsifikasi Skeleton Aksial dan Kemampuan Autotomi Regenerat Ekor Kadal (Mabouya multifasciata Kuhl). Tesis. Yogyakarta: Universitas Gadjah Mada.
Luthfi MJ, NP Soesilo, M Sagi. 2003. Kalsifikasi Skeleton Aksial pada Regenerat Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 3 (1): 1-8.
Martel-Pelletier J, A Farran, E Montell, J Verges, JP Pelletier. 2015. Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate. Molecules 20 (3): 4277-4289.
Nain Z, MDA Islam, SH Chowdhury, S Afroza, I Hussain. 2016. Current Understanding on Tail Regeneration in Green Anoles (Anolis carolinensis). Cell Biology 4 (2): 9-17.
Narasimha GA, A Janardhan, P Kumar, MR Pradeep, DS Gopal. 2012. Wound healing property of bioactive compound from actinomycetes. Der Pharmacia Sinica 3 (5):542-545.
Narayanan A. 2015. The Initiation and Progression of Tail Regeneration in Northern House Gecko Hemidactylus Flaviviridis at Role of Fibroblast Growth Factor 2 (Fgf2). Journal Biochip and Tissue Chip 5 (1): 1-7.
Naya DE, C Veloso, LP José, Muñoz and Francisco Bozinovic. 2007. Some vaguely explored (but not trivial) costs of tail autotomy in lizards. Comparative Biochemistry and Physiology 146: 189–193.
Patnaik N, C Mahapatra, SK Dutta, PK Mahapatra. 2012. Role of Acid and Alkaline Phosphatase During Vitamin A Induced Abnormal Tail Regeneration in the Tadpoles of the Indian Tree Frog. International Journal of Current Research 4 (11): 115-120.
Rosas-cruz GP, CR Silva-Correa, AA Calderón-Peña, VE Villarreal-La Torre, CL Aspajo-Villalaz, JL Cruzado-Razco, JD Rosario-Chávarri, JC Rodríguez-Soto, OE Pretel-Sevillano, WAS Guarniz, AD González-Siccha. 2020. Wound Healing Activity of an Ointment from Solanum tuberosum L. "Tumbay Yellow Potato" on Mus musculus Balb/c. Pharmacognosy Journal 12 (6): 1268-1275.
Rutland CS, P Cigler and V Kubale. 2019. Reptilian Skin and Its Special Histological Structures. In: Rutland C and V Kubale. Veterinary Anatomy and Physiology. University of Nottingham, Great Britain.
Sinclair JW, DR Hoying, E Bresciani, DD Nogare, CD Needle, A Berger, W Wu, K Bishop, AG Elkahloun, A Chitnis, P Liu, and SM Burgess. 2021. The Warburg effect is necessary to promote glycosylation in the blastema during zebrafish tail regeneration. Regenerative Medicine 6 (55): 1-16.
Soesilo NP. 1982. Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl) Setelah Mengalami Autotomi. Tesis. Yogyakarta: Universitas Gadjah Mada.
Soesilo NP. 1992. Proses Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 1 (4): 169-175.
Soesilo NP. 1999. Peranan Lapisan Ependima Dalam Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 2 (8): 419-450.
Soesilo NP. 2002. Pengaruh Regenerat Ekor Kadal (Mabouya multifasciata Kuhl.) Terhadap Angiogenesis. Berkala Ilmiah Biologi 2 (14): 833-843.
Sulityowati W, TA Indhira, A Arbai, Yatmasari. 2015. Glucosamine and Chondroitin Sulphate Content of Shark Cartilage (Prionace glauca) and its Potential as Anti-Aging Supplements. International Journal of Chemical Technology Research 8 (10): 163-168.
Vasiliadis HS and K Tsikopoulos, 2017. Glucosamine and chondroitin for the treatment of osteoarthritis. World Journal of Orthopedics 8 (1): 1-11.
Vitt LJ, JD Congdon, and NA Dickson. 1977. Adaptive Strategies and Energetics of Tail Autotomy in Lizards. Ecology 58 (2): 326-337.
Wolff RB. 2014. Glucosamine and chondroitin sulfate association increases tibial epiphyseal growth plate proliferation and bone formation in ovariectomized rats. Clinics 69 (12): 1-7.
Wu P, L Alibardi and CM Chuong. 2014. Regeneration of reptilian scales after wounding: neogenesis, regional difference, and molecular modules. Regeneration 1 (1): 15-26.
Zhang QW, LG Lin and WC Ye. 2018. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine 13 (20): 1-26.
Alibardi L. 2017. FGFs Treatment on Amputated Lizard Limbs Stimulate the Regeneration of Long Bones, Opening New Avenues for Limb Regeneration in Amniotes: A Morphological Study. Journal of Functional Morphology and Kinesiology 2 (25): 1-17.
Albardi L and VB Meyer-Rochow. 2021. Regeneration in Reptiles Generally and the New Zealand Tuatara in Particular as a Model to Analyse Organ Regrowth in Amniotes: A Review. Journal of Developmental Biology 9 (36): 1-21.
Al-Saadi HM, K Lun-Pang, SI Nirwana, K Yong-Chin. 2019. Multifaceted Protective Role of Glucosamine against Osteoarthritis: Review of Its Molecular Mechanisms. Scientia Pharmaceutica 87 (34): 1-18.
Apriyanto P, AH Yanti, TR Setyawati. 2015. Keragaman Jenis Kadal Sub Ordo Sauria pada Tiga Tipe Hutan di Kecamatan Sungai Ambawang. Protobiont 4 (1): 108-114.
Avery RA. 1970. Utilization of caudal fat by hibernating common lizards, Lacerta vivipara. Comp. Biochem. Physiol 37: 119-121.
Bargahi A and A. Rabbani-Chadegani A. 2008. Angiogenic inhibitor protein fractions derived from shark cartilage. Journal Biochemical Society 28 (1): 15–21.
Borba FKSL, EVL Costa, VAB Polli, DS Coelho, M Maraschin, PF Dias and RA Nogueira. 2017. Pro-angiogenic Activity Assay of Chondroitin Sulfate and Glucosamine Sulfate on Vascular Network of Mouse and of Chick Embryo Chorioallantoic Membrane. Journal of Glycobiology 6 (3): 1-6.
Chang C, P Wu, RE Baker, PK Maini, L Alibardi, and CM Chuong. 2009. Reptile scale paradigm: Evo-Devo, pattern formation, and regeneration. The International Journal of Developmental Biology 53: 813-826.
Dean MN and AP Summers. 2006. Mineralized Cartilage in the skeleton of chondrichthyan fishes. Zoology 109 (2): 164-168.
Delorme SL, IM Lungu and MK Vickaryous. 2012. Scar-Free Wound Healing and Regeneration Following Tail Loss in the Leopard Gecko, Eublepharis macularius. The Anatomical Record 295 (10):1575–1595.
Ferreti P, F Zhang, LS Ruis, and JDW Clarke. 2001. FGF signalling and blastema growth during amphibian tail regeneration. International Journal Development Biology (45): 127-128.
Fisher RE, LA Geiger, LK Stroik, ED Hutchins, RM George, DF Denardo, K Kusumi, J Alan Rawls and J Wilson-Rawls. 2012. A Histological Comparison of the Original and Regenerated Tail in the Green Anole, Anolis carolinensis. The Anatomical Record 295 (10):1609–1619.
Gilbert RWD, MK Vickaryou,s and AM Viloria-Petit. 2013. Characterization of TGFb Signaling During Tail Regeneration in the Leopard Gecko (Eublepharis macularius). Developmental Dynamics 242:886–896.
Greer AE and DG Broadley. 2000. Six Characters of Systematic Importance In The Scincid Lizard Genus Mabuya. Hamadryad 25 (1): 1-12.
Hartomo BT, FG Firdaus. 2014. Pemanfaatan Biomaterial Kitosan Dalam Bidang Bedah Mulut. Jurnal Kedokteran Gigi Universitas Baiturrahmah 6 (1): 63-70.
Hutchins ED, Wilson-Rawls, and Kusumi. 2016. Innovations in Molecular Mechanisms and Tissue Engineering, Stem Cell Biology and Regenerative Medicine. Springer International Publishing Switzerland. Arizona State University, Phoenix. The USA. P:23-35.
Inayah N, NP Soesilo and R Pratiwi. 2017. Effect of Tokay Gecko (Gekko gecko LINNAEUS, 1758) Saliva on Angiogenesis During Wound Healing Phase of Autotomized Tail in Common Sun Skink (Eutropis multifasciata KUHL, 1820. Jurnal Biologi Indonesia 13 (2): 253-260.
Jacyniak K, RP McDonald, MK Vickaryous. 2017. Tail regeneration and other phenomena of wound healing and tissue restoration in lizards. Journal of Experimental Biology 220 (16): 2858-2869.
Jagnandan K, AP Rus,sell and TE Higham. 2014. Tail autotomy and subsequent regeneration alter the mechanics of locomotion in lizards. The Journal of Experimental Biology (217):3891-3897.
Jerosch J. 2011. Effects of Glucosamine and Chondroitin Sulfate on Cartilage Metabolism in OA: Outlook on Other Nutrient Partners Especially Omega-3 Fatty Acids. International Journal of Rheumatology. Page: 1-18.
Kent GC. 1987. Comparative Anatomy of the Vertebrates. Sixth edition. Times Mirror/ Mosby College Publishing. UThe nited States of America. p: 192.
Landreneau EB. 2011. Scales and Scale-Like Structures. Dissertation. College Station: Texas A&M University
Londono R, Wenzhong, Wang, RS Tuan, and TP Lozito. 2017. cartilage and Muscle cell Fate and Origins during lizard Tail regeneration. Frontiers in Bioengineering and Biotechnology 5 (70): 1-9.
Lopez-senra E, P Casal-Beiroa, M López-Álvarez, J Serra, P González, J Valcarcel, JA Vázquez, EF Burguera, FJ Blanco, and J Magalhães. 2020. Impact of Prevalence Ratios of Chondroitin Sulfate (CS)- 4 and -6 Isomers Derived from Marine Sources in Cell Proliferation and Chondrogenic Differentiation Processes. Marine Drugs 18 (94): 1-13.
Lozito TP and RS Tuan. 2017. Lizard Tail Regeneration As An Instructive Model of Enhanced Healing Capabilities In An Adult Amniote. Connective Tissue Research 58 (2): 145–154.
Luthfi MJ. 2002. Kalsifikasi Skeleton Aksial dan Kemampuan Autotomi Regenerat Ekor Kadal (Mabouya multifasciata Kuhl). Tesis. Yogyakarta: Universitas Gadjah Mada.
Luthfi MJ, NP Soesilo, M Sagi. 2003. Kalsifikasi Skeleton Aksial pada Regenerat Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 3 (1): 1-8.
Martel-Pelletier J, A Farran, E Montell, J Verges, JP Pelletier. 2015. Discrepancies in Composition and Biological Effects of Different Formulations of Chondroitin Sulfate. Molecules 20 (3): 4277-4289.
Nain Z, MDA Islam, SH Chowdhury, S Afroza, I Hussain. 2016. Current Understanding on Tail Regeneration in Green Anoles (Anolis carolinensis). Cell Biology 4 (2): 9-17.
Narasimha GA, A Janardhan, P Kumar, MR Pradeep, DS Gopal. 2012. Wound healing property of bioactive compound from actinomycetes. Der Pharmacia Sinica 3 (5):542-545.
Narayanan A. 2015. The Initiation and Progression of Tail Regeneration in Northern House Gecko Hemidactylus Flaviviridis at Role of Fibroblast Growth Factor 2 (Fgf2). Journal Biochip and Tissue Chip 5 (1): 1-7.
Naya DE, C Veloso, LP José, Muñoz and Francisco Bozinovic. 2007. Some vaguely explored (but not trivial) costs of tail autotomy in lizards. Comparative Biochemistry and Physiology 146: 189–193.
Patnaik N, C Mahapatra, SK Dutta, PK Mahapatra. 2012. Role of Acid and Alkaline Phosphatase During Vitamin A Induced Abnormal Tail Regeneration in the Tadpoles of the Indian Tree Frog. International Journal of Current Research 4 (11): 115-120.
Rosas-cruz GP, CR Silva-Correa, AA Calderón-Peña, VE Villarreal-La Torre, CL Aspajo-Villalaz, JL Cruzado-Razco, JD Rosario-Chávarri, JC Rodríguez-Soto, OE Pretel-Sevillano, WAS Guarniz, AD González-Siccha. 2020. Wound Healing Activity of an Ointment from Solanum tuberosum L. "Tumbay Yellow Potato" on Mus musculus Balb/c. Pharmacognosy Journal 12 (6): 1268-1275.
Rutland CS, P Cigler and V Kubale. 2019. Reptilian Skin and Its Special Histological Structures. In: Rutland C and V Kubale. Veterinary Anatomy and Physiology. University of Nottingham, Great Britain.
Sinclair JW, DR Hoying, E Bresciani, DD Nogare, CD Needle, A Berger, W Wu, K Bishop, AG Elkahloun, A Chitnis, P Liu, and SM Burgess. 2021. The Warburg effect is necessary to promote glycosylation in the blastema during zebrafish tail regeneration. Regenerative Medicine 6 (55): 1-16.
Soesilo NP. 1982. Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl) Setelah Mengalami Autotomi. Tesis. Yogyakarta: Universitas Gadjah Mada.
Soesilo NP. 1992. Proses Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 1 (4): 169-175.
Soesilo NP. 1999. Peranan Lapisan Ependima Dalam Regenerasi Ekor Kadal (Mabouya multifasciata Kuhl). Biologi 2 (8): 419-450.
Soesilo NP. 2002. Pengaruh Regenerat Ekor Kadal (Mabouya multifasciata Kuhl.) Terhadap Angiogenesis. Berkala Ilmiah Biologi 2 (14): 833-843.
Sulityowati W, TA Indhira, A Arbai, Yatmasari. 2015. Glucosamine and Chondroitin Sulphate Content of Shark Cartilage (Prionace glauca) and its Potential as Anti-Aging Supplements. International Journal of Chemical Technology Research 8 (10): 163-168.
Vasiliadis HS and K Tsikopoulos, 2017. Glucosamine and chondroitin for the treatment of osteoarthritis. World Journal of Orthopedics 8 (1): 1-11.
Vitt LJ, JD Congdon, and NA Dickson. 1977. Adaptive Strategies and Energetics of Tail Autotomy in Lizards. Ecology 58 (2): 326-337.
Wolff RB. 2014. Glucosamine and chondroitin sulfate association increases tibial epiphyseal growth plate proliferation and bone formation in ovariectomized rats. Clinics 69 (12): 1-7.
Wu P, L Alibardi and CM Chuong. 2014. Regeneration of reptilian scales after wounding: neogenesis, regional difference, and molecular modules. Regeneration 1 (1): 15-26.
Zhang QW, LG Lin and WC Ye. 2018. Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine 13 (20): 1-26.
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Published
2022-02-22
How to Cite
Rakhmiyati, Widiyani, T. ., & Budiharjo, A. . (2022). Histological Structure of Regenerate Tail in Lizard (Mabouya multifasciata Kuhl) After Treatment Using Shark Cartilage Ointment. Proceeding International Conference on Religion, Science and Education, 1, 527–536. Retrieved from http://sunankalijaga.org/prosiding/index.php/icrse/article/view/833
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