Analysis of Endo-1,4-?-Glucanase (eglS) Gene in Cellulose-Degrading Bacteria (Bacillus) Based on In Silico Research

Authors

  • Umi Uswatun Hasanah UIN Sunan Kalijaga Yogyakarta
  • Safitri Anggita Tunjung Sari UIN Sunan Kalijaga Yogyakarta
  • Jumailatus Solihah UIN Sunan Kalijaga Yogyakarta

Keywords:

Keywords : Bacillus, Homology eglS gen, Cellulose degradation

Abstract

Abstract. The process of cellulose degradation naturally requires the help of microorganisms. The group of Bacillus bacteria is one of the cellulolytic bacteria that can break down cellulose. The mechanism of cellulose degradation in Bacillus is a complex system and was regulated by a number of genes. One of Bacillus genes that has mechanism of cellulose degradation is eg1S gene. This gene is responsible to hydrolyze the internal glycosidic bonds to reduce the long chain of cellulose. This research examines the comparasion of endo-1,4-?glucanase gene homology in Bacillus that can degrade cellulose. The method of research is in sillico-based research by using National Center for Biotechnology Information (NCBI) website, Clustal Omega website, and MEGA-X software. The results obtained in this research are the identity of endo-1,4-?-glucanase gene, phylogenetic tree, and similarity index in Bacillus that can degrade cellulose. From the research that has been done, it can be concluded that the highest homology ratio of endo-1,4-?-glucanase gene is occupied by Bacillus subtilis strain AuChE413 and Bacillus subtilis strain IARI-SP-1 with 100% identical similarities. 

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References

Arifin, Z., Gunam , I. B., Antara, N. S., & Setiyo, Y. 2019. Isolasi Bakteri Selulolitik Pendegradasi Selulosa dari Kompos. Jurnal Rekayasa dan Manajemen Agroindustri, 7(1) : 3037. Blackshelds, G., & Wallace , I. M. 2006. Analysis and comparison of benchmark for multiple sequence alignment. Silico Bio, 6(6) : 321-339. Faiqoh, H. 2016. Efisiensi Hidrolisis Tepung Kulit Ubi Kayu Menggunakan Aspergilus niger dan Trichoderma viride. Jember: Fakultas Teknologi Pertanian Jember. Hatmanti, A. 2000. Pengenalan Bacillus Spp. Oseana, 25(1) : 31-41. Irfan, M. S., Syed, Q., & Nadeem , M. 2012. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turkish Journal of Biochemistry, 37(3) : 287-293. Jayus, J., Nafi, A., & Hanifa, A. S. 2019. Degradasi Komponen Selulosa, Hemiselulosa, Dan Pati Tepung Kulit Ubi Kayu Menjadi Gula Reduksi Oleh Aspergilus niger, Trichoderma viride, dan Acremonium sp. IMI 383068. Jurnal Agroteknologi, 13(1) : 34-41.
Kurniawan , A., Sari, S. P., Asriani, E., Kurniawan, A., Sambah, A. B., Triswiyana, I., & Prihanto, A. A. 2019. Kapasitas Hidrolisis Bakteri Pendegradasi Selulosa Dari Ekosistem Mangrove. Journal of Tropical Marine Science, 2(2) : 76-82. Laderia, S. A., Cruz, E., Delatorre, A. B., Barbosa, J. B., & Martins, M. L. 2015. Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility. Electronic Journal of Biotechnology, 18 : 110-115. Mount, D. W. 2001. Phylogenetic prediction In Bioinformatics, Sequens and Genome Analysis. Cold Spring Habor Laboratory, 5(6) : 237-280. Mulyasari, Wardani, Suprayudi, M. A., Junior, M. Z., & Sunarno, M. T. 2015. Seleksi dan Identifikasi Bakteri Selulolitik Pendegradasi Daun Singkong (Manihot esculenta) yang Diisolasi dari Saluran Pencernaan Ikan Gurame ( Ospronemus gouramy). JPB Kelautan dan Perikanan, 10(2) : 111-121. Nofu, K., Khotimah, S., & Lovadi, I. 2014. Isolasi dan Karakterisasi Bakteri Pendegradasi Selulosa Pada Ampas Tebu Kuning (Baggase). Jurnal Protobion, 1(5) : 25-33. Sitou, N., & Mei, M. 2009. The Neighbour-Joinning Method : A new method for contructing phylogenetic trees. Molecular Biology, 6(5) : 514-525. Ulfa, A., Khotimah, S., & Linda, R. 2014. Kemampuan Degradasi Selulosa oleh Bakteri Selulolitik yang Diisolasi dari Tanah Gambut. Protobioint, 3(2) : 259-267. Zhang, X. Z., & Zhang, Y. H. 2013. Cellulases: Characteristics, Sources, Production and Applications. Bioprocessing Technologies. New York: John Wiley & Sons, Inc.

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Published

2021-02-27

How to Cite

Hasanah, U. U., Sari, S. A. T., & Solihah, J. (2021). Analysis of Endo-1,4-?-Glucanase (eglS) Gene in Cellulose-Degrading Bacteria (Bacillus) Based on In Silico Research. Proceeding International Conference on Science and Engineering, 4, 80–83. Retrieved from http://sunankalijaga.org/prosiding/index.php/icse/article/view/625

Issue

Vol. 4 (2021)

Section

Articles