Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage
Keywords:
Keywords: Acclimatization; Environment condition; Media; Plantlets; Tissue cultureAbstract
Abstract. Tissue culture is a method of propagation plants by isolating cell, tissues, protoplasts or plant organs than planting them in a sterile and controlled environment. This method is able to produce superior plants and propagation of plants are difficult to cultivate conventionally in large quantities with a relatively fast time. Acclimatization is an important final stage in the success of tissue culture method. Acclimatization is carried out for adaptation of the plantlet environment from in vitro to in vivo conditions. This is caused in the previous stage the plantlets are heterotrophic, while on acclimatization stage it will be autotrophic. This study aims to provide an information about the factors that affect the success of acclimatization plantlets. This is a review article was conducted using the literature study method by collecting information from journals, books or internet than analyzed in descriptive qualitative to prove scientifically. On this stage, many plantlets fail to grow and even die. for increase growth and reduce plantlets mortality need to be made overcome these problems. This can be overcome by applying acclimatization supporting factors. The result that factors affecting of the success plantlet growth on acclimatization stage including: the condition of the plantlets, light intensity, temperature, relative humidity, types of planting media, nutrition and fertilizing and controlling of pest and microorganisms.
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References
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Wahyuningsih, Tri. 2016. Pengaruh intensitas cahaya terhadap keberhasilan aklimatisasi embrio somatik kopi robusta (Coffea canephora Pierre ex A. Froehner) secara langsung [Bachelor thesis] Universitas Muhammadiyah Purwokerto. Purwokwrto. Wijayanto, T., and Boer, D. 2013. Kemajuan tahapp aklimatisasi planlet kedelai (Glycine max) hasil kultur in vitro immature embrio. Agriplus 22: 72–76.
Yosepa, T., Siregar, C., and Gusmayanti, E. 2013. Pengaruh penggunaan jenis media terhadap aklimatisasi anggrek Dendrobium Sp (Hibrida). Dissertation. [Tanjungpura University]. Pontianak Zuklarnain. 2018. Kultur jaringan tanaman. Bumi aksara: Jakarta.
Cha-um, S., Ulziibat, B., and Kirdmanee, C. 2010. Effects of temperature and relative humidity during in'vitro'acclimatization, on physiological changes and growth characters of' Phalaenopsis' adapted to in'vivo'. Australian Journal of Crop Science. 4: 750756 Darwesh, R. S. 2015. Morphology, physiology and anatomy in vitro affected acclimatization ex vitro date palm plantlets: A Review. International Journal of Chemical, Environmental & Biological Sciences 3: 183-190. Dinarti, D., Sayekti, U., & Alitalia, Y. 2010. Tissue culture of pitcher plant (Nepenthes mirabilis). Jurnal Hortikultura Indonesia. 1: 59-65. Dwiyani, R. 2014. Respon pertumbuhan bibit anggrek Dendrobium sp. pada saat aklimatisasi terhadap beragam frekuensi pemberian pupuk daun. Agrotrop: Journal on Agriculture Science 2: 171175. Fauziah, A. A., Prahasti, N., Setiari, N., Saptiningsih, E. 2020. Shade and Different Substrate Types of Phalaenopsis Hybrid Ex-vitro Acclimatization Period. Bulletin of Anatomy and Physiology 5: 60-66. Febriyani, S., Ganefianti, D. W., Romeida, A., and Herawati, R. 2019. Acclimatization of pencil orchid (Papillionanthe hookeriana Rehb. f) as affected by different types of planting media and fertilizing frequency. Akta Agrosia.22: 36-41. Husen, S. 2008. Optimasi media pengakaran dan aklimatisasi planlet bibrid mangga. Jurnal Tropika. 16: 34-39. Irsyadi, M. B. 2018. Culture in vitro method on Gerbera jamesonii. [Internship report unpublished]. Universitas Muhammadiyah Yogyakarta, Yogyakarta [Indonesia]. Julhendri, J., Gultom, H., & Fathurrahman, F. 2013. Acclimatization of anthurium (Athurium sp.) with various growth media and growquick fertilizer. Dinamika Pertanian, 28: 103-112. Kang, H., Kang, K. W., Kim, D. H., dan Sivanesan, I. 2020. In Vitro Propagation of Gastrochilus matsuran (Makino) Schltr., an Endangered Epiphytic Orchid. Plants 9: 524-531. Kasutjianingati, K., Firgiyanto, R., and Warisu, A. E. 2020. Response of the vanda plantlet (Vanda sanderiana) to the addition of guano and mycorrhizal fertilizers in the acclimatization stadia. IOP Conference Series: Earth and Environmental Science. DOI:10.1088/1755-1315/411/1/012005. Kasutjianingati, K., Firgiyanto, R., dan Firnawati, L. 2020. The Growth of Vanda Seedling (Vanda sanderiana) with some Types of Plant Media Acclimatization and Concentration of Humic Acid. IOP Conference Series: Earth and Environmental Science. DOI:10.1088/1755-1315/411/1/012003. Kurniasih, W., Nabiila, A., Karimah, S. N., Fauzan, M. F., Riyanto, A., & Putra, R. R. 2017. Utilization of zeolite as a media of acclimatization to optimize growth of phalaenopsis sp. hybrid. Bioma: Jurnal Ilmiah Biologi. DOI:10.26877/bioma.v6i2.1713 Latif, R. A., Hasibuan, S., and Mardiana, S. 2020. Stimulasi pertumbuhan dan perkembangan planlet anggrek (Dendrobium sp) pada tahap aklimatisasi dengan pemberian vitamin B1 dan atonik. Jurnal Ilmiah Pertanian. 2:127-134. Maera, Z., Yusnita, Y., & Susriana, S. 2014. Respon pertumbuhan planlet anggrek phalaenopsis hibrida terhadap pemberian dua jenis pupuk daun dan benziladenin selama aklimatisasi. Enviagro: Jurnal Pertanian dan Lingkungan, 7: 33–38. Malabadi RB, Kumar SV, Mulgund GS, Nataraja K. 2011. Induction of somatic embryogenesis in papaya (Carica papaya L.). Res Biotechnol 2: 40-55. Martins, J. P. R., Schimildt, E. R., Alexandre, R. S., Falqueto, A. R., end Otoni, W. C. 2015. Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels. In Vitro Cellular & Developmental Biology-Plant. DOI:10.1007/s11627-015-9711-z Mulyono, D. 2014. Pengaruh intensitas naungan dalam aklimatisasi pertumbuhan planlet gaharu (Aquilaria beccariana). Jurnal Sains dan Teknologi Indonesia. 16 DOI:10.29122/jsti.v16i1.3404
Nasution, L. Z., Hasibuan, M., and Manurung, E. D. 2020. Adaptability of tissue-cultured Dendrobium orchid planlets on planting media and its position during acclimatization process. E&ES. DOI:10.1088/1755-1315/454/1/012166 Nisa, C. and Rodinah, R., 2018. Kultur Jaringan beberapa Kultivar Buah Pisang (Musa paradisiaca L.) dengan Pemberian Campuran NAA dan Kinetin. BIOSCIENTIAE, 2 (2). Pérez, L. P., Montesinos, Y. P., Olmedo, J. G., Rodriguez, R. B., Sánchez, R. R., Montenegro, O. N., Escriba, R. C. R., Daniels, D., and Gómez-Kosky, R. 2016. Effect of phloroglucinol on rooting and in vitro acclimatization of papaya (Carica papaya L. var. Maradol Roja). In Vitro Cellular & Developmental BiologyPlant. DOI: 10.1007/s11627-015-9733-6 Pinar, H., K?l?nc, N., and Uzun, A. 2020. Effect of different temperature and moisture on development of in vitro derived banana plantlets. Current Trends in Natural Sciences. 9: 216-221. Rachmawati, F., Pramanik, D., and Winarto, B. 2020. Adventitious shoots derived from leaf explants in in vitro mass propagation of Indonesian selected Anthurium clones. International Journal of Agriculture Forestry and Life Sciences. 4: 48-55. Riyadi, I. 2016. Criteria of kopyor coconut plantlets ready to be acclimatized. E-Journal Menara Perkebunan. DOI:10.22302/iribb.jur.mp.v84i1.203 Rodinah, R., Razie, F., Nisa, C., and Hardarani, N. 2015. Efek Komposisi Media Tanam dan Jenis Pupuk Daun terhadap Keberhasilan Aklimatisasi Pisang Talas (Musa paradisiaca Var. Sapientum L.). In Prosiding Seminar Nasional FKPTPI 2015. Universitas Lambung Mangkurat, Banjarbaru, 29-30 September 2015. [Indonesia] Romodhon, S. 2017. Pengaruh berbagai media tanam terhadap aklimatisasi anggrek Dendrobium sp. Undergraduate thesis. [Universitas Medan Area]. Medan. Royani, J. I. 2014. Pengaruh media aklimatisasi pada pertumbuhan dan umbi keladi tikus; Prossiding Seminar Nasional Aspek Budaya, Kebijakan Dan Filosofi Sains Jamu, IPB University, Bogor, Oktober 2014. [Indonesia] Siburian, M. F., & Damayanti, F. 2016 aklimatisasi planlet tebu ps 864 pasca enkapsulasi; Prosiding seminar tahunan matematika, sains dan teknologi, Universitas Terbuka, Jakarta, 22 September 2016. [Indonesia] Sisunandar, S. 2014. Produksi bibit kelapa kopyor true-to-type melalui teknik kultur embryo; Prosiding Seminar Nasional XI Pendidikan Biologi FKIP UNS, Surakarta, 7 Juny 2014. [Indonesia] Sukmadijaya, D., Dinarti, D., and Isnaini, Y. 2013. Pertumbuhan planlet kantong semar (Nepenthes rafflesiana Jack.) pada beberapa media tanam selama tahap aklimatisasi. Jurnal Hortikultura Indonesia, 4: 124-130. Sumaryono, S., and Riyadi, I. 2011. Ex vitro rooting of oil palm (Elaeis guineensis Jacq.) plantlets derived from tissue culture. Indonesian Journal of Agricultural Science 12: 57-62. Sumaryono, Sinta, M. M., Nurhaimi-Haris. 2016. Survival rate of in vitro microcutting-derived rubber plantlets on various plastic cover closed periods and medium compositions. E-Journal Menara Perkebunan 80: 25-31. Sutthinon, P., Pan-aon, K., Meesawat, U., Jantasilp, A., Danaee, E., Saljoqi, A. U. R., and Huma, Z. 2015. Acclimatization of in vitro germinated seedlings of tiger orchid (Grammatophyllum speciosum Blume.) in hydroponic culture using dynamic root floating technique (DRFT) with chitosan spraying. Thai Journal of Agricultural Science. 48: 47-53. Taryono. 2014. Pengantar bioteknologi untuk pemuliaan tanaman. Gadjah Mada Press, Yogyakarta. Valero-Aracama, C., Kane, M. E., Wilson, S. B., Vu, J. C., Anderson, J., & Philman, N. L. 2006. Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during in vitro culture and ex vitro acclimatization. In Vitro Cellular & Developmental BiologyPlant 42: 572-583.
Wahyuningsih, Tri. 2016. Pengaruh intensitas cahaya terhadap keberhasilan aklimatisasi embrio somatik kopi robusta (Coffea canephora Pierre ex A. Froehner) secara langsung [Bachelor thesis] Universitas Muhammadiyah Purwokerto. Purwokwrto. Wijayanto, T., and Boer, D. 2013. Kemajuan tahapp aklimatisasi planlet kedelai (Glycine max) hasil kultur in vitro immature embrio. Agriplus 22: 72–76.
Yosepa, T., Siregar, C., and Gusmayanti, E. 2013. Pengaruh penggunaan jenis media terhadap aklimatisasi anggrek Dendrobium Sp (Hibrida). Dissertation. [Tanjungpura University]. Pontianak Zuklarnain. 2018. Kultur jaringan tanaman. Bumi aksara: Jakarta.
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2021-02-27
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Irsyadi, M. B. (2021). Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage. Proceeding International Conference on Science and Engineering, 4, 100–104. Retrieved from http://sunankalijaga.org/prosiding/index.php/icse/article/view/632
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