Sugar Residues and Their Variations of Distribution on Ovarian Follicles of Timor Deer (Cervus timorensis)
Keywords:
sugar residues, follicle, Timor deer (Cervus timorensis), lectinAbstract
Timor deer is one of Indonesian tropic deer has not most which investigated in Indonesia on biology of reproduction. The data of sugar residues containing on ovarian follicle of timor deer are important to support application of reproduction technology as effort and to increase timor deer utility as livestock with maximal quality.Ovaries isolated from 2 timor deers were fixed with bouin’s and embedded in paraffin wax. Sections 5 µm, were deparaffined, rehydrated and labelled with four different lectins (PHA, RCA, Con A, and LCA). Preantral follicle has founded carbohydrate with D-N-acetylgalactosamine, galactose and mannose sugar residues in cytoplasm and zona pellucida oocyte, granulosa and teca cells. The antral follicle has not founded mannose in oocyte cytoplasm. D-N-acetylgalactosamine, galactose, mannose and glucose sugar residues have founded in zona pellucida, folicular liquid, granulosa and teca cells.