Green Tea Extract Protects Endothelial Progenitor Cells from Oxidative Damage Through Reduction of Intracellular Reactive Oxygen Species Activity

Authors

  • Wahyu Widowati Maranatha Christian University
  • Rahma Micho Widyanto Brawijaya University
  • Dian Ratih Laksmitawati Pancasila University Jakarta
  • Winsa Husin Maranatha Christian University
  • Hana Ratnawati Maranatha Christian University
  • Indra Bachtiar Stem Cell and Cancer Institute, Jakarta

Keywords:

endothelial progenitor cell, oxidative stress, reactive oxygen species, green tea, antioxidant, endothelium, coronary diseases

Abstract

A number studies have examined that tea consumption decreases cardiovascular risk, but the mechanisms remain undefined. Endothelial dysfunction has been correlated with coronary artery disease and circulating endothelial progenitor cells (EPCs) is contributed of this repair process. Endothelial dysfunction is associated with increased oxidative stress and may be reverse by antioxidant. Green tea is known as free radical scavenger which has a powerful antioxidant action. The aim of this study is to investigate whether green tea extract (GTE) can protect EPCs from oxidative stress through antioxidant protective mechanism. Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation. The cells were then plated on fibronectin-coated culture dishes. After being cultured for 7 d, EPCs were characterized as adherent cells double positive for DiLDL-uptake and lectin binding. Further characterizations were done by demonstrating the expression of CD34/45, CD133, and KDR. EPCs were then induced for oxidative stress using various concentrations of H2O2  (50, 100, 200 µM), and incubated with or without GTE (25 mg/L) and result showed that GTE ameliorated the cell viability of H202-induced EPC at concentration 50, 100, 200 µM for about 28.72 ± 10.5%, 34.55 ± 7.64%, and 27.04 ± 3.42%, respectively,  higher  than that control. The  level  of  intracellular  reactive  oxygen  species (ROS) was quantified by fluorescence with 2’,7’- dichlorofluorescein diacetate (DCFH-DA) using flow cytometry and  showed  that GTE  decreased intracellular ROS level of  H202- induced EPC at concentration 50, 100, 200 µM for about 84.24 ± 8.59 %, 92.27 ± 1.08 %, and 93.72 ± 0.36%, respectively, compare to that control. The result showed that GTE may ameliorate cell viability by decreasing accumulation of intracellular ROS in H202-induced EPCs.

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Published

2011-10-31

How to Cite

Widowati, W. ., Widyanto, R. M. ., Laksmitawati, D. R. ., Husin, W. ., Ratnawati, H. ., & Bachtiar, I. . (2011). Green Tea Extract Protects Endothelial Progenitor Cells from Oxidative Damage Through Reduction of Intracellular Reactive Oxygen Species Activity. Proceeding ICBB (The International Conference on Bioscience and Biotechnology), 1(1), B44-B55. Retrieved from https://sunankalijaga.org/prosiding/index.php/icbb/article/view/168

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Articles