Potential Cytotoxic on Breast Cancer Cells Line and Antioxidant of Water Extract of Catharanthus roseus [L] G.Don., Dendropthoe petandra L., Curcuma mangga Val., Piper betle L.
Keywords:
cytotoxic, antioxidant, Catharanthus roseus, Dendropthoe petandra, Curcuma mangga, Piper betle, water extract, breast cancer, T47DAbstract
Breast cancer is the most common cancer among women and the second leading cause of cancer deaths in women today. The madagascar prewinkle (Catharanthus roseus [L] G.Don), mango parasite (Dendropthoe petandra L.), white saffron (Curcuma mangga Val), betel leaves (Piper betle L.) have been reported to exhibit antioxidant, antimutation and cytotoxic that suggested the chemopreventive potential against various cancer including breast cancer. This research was conducted to investigate cytotoxic activity on breast cancer cell line T47D, antioxidant activity of C. roseus, D. petandra, C. mangga and P. betle water extracts. The cytotoxic potency was determined with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The antioxidant activities were determined by using in vitro assay of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. C. roseus water extract was able to inhibit T47D cell proliferation with IC50 4%, D. petandra with IC50 1%, C. mangga with IC50 14% and P. betle with IC50 3%. The highest DPPH scavenging activity of C. roseus was 71.87%, D. petandra was 75.11%, C. mangga was 38.45% and P. betle water extract was 83%. We suggest that D. petandra, P. betle and C. roseus water extract have a potential cytotoxic and antioxidant activities compared with C. mangga water extract.