Cytotoxic Activity Prescreen of Leaves of Primate Consumed Plants Subclassis dilleniidae and hamamelididae Using Brine Shrimp Lethality Test
Keywords:
Brine Shrimp Lethality Test (BSLT), cytotoxic, primate consumed plant, Dilleniidae and Hamamalididae, TangkoloAbstract
Brine Shrimp Lethality Test (BSLT) is a prescreened method of cytotoxic activity which one of its aim is finding a new cancer agent. The closest relatives between human and nonhuman primate emerge an idea to utilize a primate consumed plant as an alternative medicinal. In this experiment, five plants extracts from each subclassis (Dilleniidae and Hamamalididae) were tested using BSLT. The result showed that nine of ten extract were toxic (LC50 < 1000 µg/mL) and the highest activity was given by Tangkolo (Kleinhovia hospita) extract whose LC50 was 88,025 µg/mL. Furthermore, Tangkolo extract was fractionated by Liquid-Liquid Extraction using n-hexane, ethyl acetate and water. All fractions then tested by BSLT. The result showed that ethyl acetate fraction gave the highest activity. The LC50 value of n-hexane fraction, ethyl acetate fraction and water fraction respectively were 93,998 µg/mL, 53,333 µg/mL and 757,424 µg/mL.