Amplification and Sequencing Growth Hormone Genes in the Nurseri Center for PO Cattle on Balai Besar Inseminasi Buatan (BBIB) Singosari and Unit Perusahaan Aliansi (UPA) Pasuruan

Authors

  • Mariana Rengkuan Manado State University
  • Aloysius Duran Corebima Malang State University
  • Sutiman Bambang Sumitro Brawijaya University
  • Mohamad Amin Malang State University

Keywords:

PO cattle, growth hormone gene, PCR, sequencing

Abstract

This study aims to amplify the bovine growth hormone gene and learn the sequence of bases as well as the homology of growth hormone gene from the gene bank. This is important because as a Indonesia local cattle in this study will be a sperm-producing parental stock which will then be used by the farmers through insemination techniques. Related to our research goal, DNA sampels of 3 superior bull PO at BBIB Singosari, East Java and 10 PO cow of the parental stock at UPA Pasuruan, East Java had been analyzed. DNA isolation had been conducted by salting out technique on the total samples, followed by PCR-sequecing procedure. The process of amplification of bovine growth hormone gene carried by one pair of primer. The process of DNA amplification by PCR  generating 329 bp long.  Results showed that correct amplicon sequencing is the bovine growth hormone gene showed 99% homology with the GH gene Bos indicus Breed Butane that has been published in GenBank.

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Published

2011-10-31

How to Cite

Rengkuan, M. ., Corebima, A. D. ., Sumitro, S. B. ., & Amin, M. . (2011). Amplification and Sequencing Growth Hormone Genes in the Nurseri Center for PO Cattle on Balai Besar Inseminasi Buatan (BBIB) Singosari and Unit Perusahaan Aliansi (UPA) Pasuruan. Proceeding ICBB (The International Conference on Bioscience and Biotechnology), 1(1), C29-C34. Retrieved from https://sunankalijaga.org/prosiding/index.php/icbb/article/view/176

Issue

Vol. 1 No. 1 (2011)

Section

Articles