Amplification and Sequencing Growth Hormone Genes in the Nurseri Center for PO Cattle on Balai Besar Inseminasi Buatan (BBIB) Singosari and Unit Perusahaan Aliansi (UPA) Pasuruan
Keywords:PO cattle, growth hormone gene, PCR, sequencing
This study aims to amplify the bovine growth hormone gene and learn the sequence of bases as well as the homology of growth hormone gene from the gene bank. This is important because as a Indonesia local cattle in this study will be a sperm-producing parental stock which will then be used by the farmers through insemination techniques. Related to our research goal, DNA sampels of 3 superior bull PO at BBIB Singosari, East Java and 10 PO cow of the parental stock at UPA Pasuruan, East Java had been analyzed. DNA isolation had been conducted by salting out technique on the total samples, followed by PCR-sequecing procedure. The process of amplification of bovine growth hormone gene carried by one pair of primer. The process of DNA amplification by PCR generating 329 bp long. Results showed that correct amplicon sequencing is the bovine growth hormone gene showed 99% homology with the GH gene Bos indicus Breed Butane that has been published in GenBank.