Isolation and Identification of Rhizoctonia Associated with Phalaenopsis amabilis (L.) Blume Roots

Abstract

Most of orchid mycorrhizal fungi are from Rhizoctonia genus and these fungi have known can enhance the growth of orchid. This study aimed to isolate and identify Rhizoctonia associated with Phalaenopsis amabilis roots. Isolation of Rhizoctonia from healthy roots was carried out using a modification of Yuan method.  Identification was based on the macroscopic and microscopic characteristics. Nuclei were stained with safranin O and KOH. Four Rhizoctonia isolates isolated from root of Ph. amabilis were identified as Ceratorhiza sp1, Ceratorhiza sp2, Epulorhiza repens, and Moniliopsis sp. Nuclear staining revealed that Ceratorhiza sp1, Ceratorhiza sp2 and Epulorhiza repens were binucleate and Moniliopsis sp. was multinucleate.