The Effectiveness of Taro Leaf Stalk (Colocasia esculenta L.) Ointment Extract on Burn Wound Healing in mice (Mus musculus L.)
Keywords:
Extract Ointment, Colocasia esculenta L., Mus mmusculus L., Burn Healing.Abstract
The purpose of this study was to determine the effectiveness of taro leaf stalk extract ointment (Colocasia esculenta L.) on the length of time for wound healing, burn diameter, and percentage of burn healing in mice (Mus musculus L.). Taro leaf stalk extraction using the Maceration method. This type of research is experimental with a completely randomized design (RAL) method with 20 male mice divided into 5 treatment groups, namely Negative Control (Ointment Base), Positive Control (Bioplacenton 10%), K1 (Taro Leaf Stalk Extract Ointment 4%), K2 (Taro leaf stalk extract ointment 6%) and K3 (Taro leaf stalk extract ointment 8%), each with 4 replications. Making burns on the backs of mice with hot metal coins with a diameter of 20 mm which were affixed for 2 seconds. Observation parameters are the length of healing time, wound diameter, and percentage of burn healing for 18 days. Data were analyzed using One Way ANOVA (Analysis Of Variance) (? = 0.05%) and continued with the LSD (Least Significant Different) test. The results showed that taro leaf stalk extract ointment with a concentration of 8% was more effective in accelerating wound healing with an average healing time of 16 days compared to positive control (16.5 days), 4% ointment (17.25 days), 6% ointment (17 days) and negative control (18 days). The diameter of the burn which was initially 20 mm the fastest was 8% ointment with a wound diameter of 0.37 mm and a high burn healing rate of 98.12% when compared to Bioplacenton 10% followed by taro leaf stalk extract ointment with a concentration of 6% and 4%.
References
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Sutrisno, T., Huda, N., Cahaya, N., & Srikartika, M. 2016. The Effectiveness of Gel Quercetin in Burn Healing Degree II. Media Pharmaceutica Indonesia, 1(1), 1-5
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Wadankar, GD, Malode, SN and Sarambekar SL 2011. Traditionally Used Medicinal Plants for Wound Healing in The Washim District, Maharashtra. IJPRIF. 3(4), 2080-2084
Wehantouw, FS, Manurung, S., Manurung., E. and Suryanto. 2011. Antihyperglycemic Activity of Mangosteen Peel Extract (Garcinia mangostana L.) in Sucrose Induced Rats. Chem. prog. 4, 89-96
Wijaya, BA, Citraningtyas, G. and Wehantouw, F. 2014. Potential of Taro Leaf Stem Ethanol Extract (Colocasia esculenta L.) as an Alternative Medicine for Rabbit Skin Wounds (Oryctolagus cuniculus). Pharmacon. 3(3), 211-21
Ansel, HC 1989. Introduction to Pharmaceutical Preparation Forms, translated by FaridaIbrahim, Edition IV. University of Indonesia Press. Jakarta.
Biren, NS, Nayak, BS, Bhatt, SP, Jalalpure, SS and Seth, AK 2007. The Anti-Inflamantory Activity of The Leaves of Colocasia esculenta. SPJ. 15(1), 3-4
Candra, S., Susilawati, E. and Adnyana, IK, 2018. Effect of Kerehau Leaf Extract Gel (Callicarpa longifolia Lam.) on Wound Healing in a Diabetic Rat Model. Pharmaceutical Scientific Journal. 6(2), 70-80
Dalimartha, S. 2005. Atlas of Indonesian Medicinal Plants. Trubus Agriwidya: Jakarta.
Dalimartha, S. 2006. Atlas of Indonesian Medicinal Plants Volume 4. Puspa Swara: Jakarta.
Faure, D. 2002. The Family-3 Glycoside Hydrolises: From Housekeeping Function to Host-microbe Interaction. Applied and Environmental Microbiology.64(4), 1485-1490
Handayani, F., Sundu, R. and Karapa, HN 2016. Activity Test of Areca Nut (Areca catechu L.) Ethanol Extract on Healing Burns on Back Skin of Male White Mice (Mus musculus). Manuntung Scientific Journal. 2(2), 11-18
Hatahet, T., Morille, M., Hommoss, A., Devoisselle, JM, Müller, RH, & Bégu, S. 2016. Quercetin topical application, from conventional dosage forms to nanodosage forms. European Journal of Pharmaceutics and Biopharmaceutics, 108.
Horton, J. A., Li, F., Chung, E. J., Hudak, K., White, A., Krausz, K. 2013. Quercetin Inhibits Radiation-Induced Skin Fibrosis Quercetin Inhibits Radiation-Induced Skin Fibrosis. BioOne Research Evolved, 180(2), 10-16
Izzati, UZ 2015. Effectiveness of Healing Burns Ointment Ethanol Extract of Senggani Leaves (Melastoma malabathricum L) on Male Rats (Rattus norvegicus) Wistar strain. Publication manuscript. Pharmacy. Medical School. Tanjungpura University. Pontianak.
Kumar, V., Abul K., Abbas, Fausto, N. and Aster, J. 2012. Tissue renewal, regeneration, and repair. Elsevier Inc. 1(1), 1212-1220
Living, L. Activity Test of Ethanol Extract of Taro Leaves (Colocasia esculenta L.) on Burn Healing in Animal Test Male Rabbits (Oryctolagus cuniculus). Thesis. Tadulako University. Hammer.http://repository.untad.ac.id/id/eprint/4580. [Accessed: April 15, 2021].
Lindawati, NY and Solikhah, A. 2018. Determination of Total Flavonoid Levels on Leaf Stalks Ethanol Extract of Taro (Colocasia esculenta L. Schott). Proceedings International Conference on Healthcare.1(1), 58-66
Maretni, S., Mukarlina and Turnip, M. 2017. Types of Taro Plants (Araceae) in Rasau Jaya District, Kubu Raya Regency. Journal of Protobiont.6(1), 42-52
Mawarsari, T. 2015. Burn Healing Activity Test of Ethanol Extract of Japanese Taro Tuber (Colocasia esculenta L. Schoot) Male Rat (Rattus norvegicus) Sprague Dawley Line. Essay. Syarif Hidayatullah State Islamic University. Jakarta.https://repository.uinjkt.ac.id/dspace/handle/123456789/37754.[Accessed: 20 February 2021].
Nasution, N. 2015. Activity Test of Ethanol Extract of Taro Tuber (Colocasia esculenta [L]. Schoot var. antiquorum) on Healing of Open Wounds in Male White Rats (Rattus norvegicus) Sprague Dawley. Essay. Syarif Hidayatullah State Islamic University. Jakarta.
Negara, RFK, Ratnawati, R. and Sli, DD 2014. Effect of Second Degree Burn Treatment Using Ethanol Extract of Betel Leaf (Piper betle Linn) on Increased Granulation Tissue Thickness in Male White Rats (Rattus norvegicus) Wistar strain. Health Magazine. 1(2), 87
Prasetyo, BFI, Wientarsih and Priosoeryanto, BP 2010. The activity of Ambon Banana Tree Stem Extract Gel Preparation in Wound Healing Process in Mice. J. Veterinary,11(2): 7073
Ramayani, SL, Sandiyani, RP and Dinastyantika, VO 2020. Effect of Differences in Plant Parts on Total Phenolic Content and Total Flavonoid Content of Taro Extract (Colocasia esculenta L.). Indonesian Pharmaceutical Media.15(2), 1611-1616
Ruswanti, E., Cholil. and Sukmana, IB 2014. The Effectiveness of Ethanol Extract of Papaya Leaves (Carica papaya) 100% on Wound Healing Time. Journal of Dentistry. 2(2), 1-5
Sangtam, TL, Jamir, NS, Deb, CR, and Amir, S. 2012. A Study on the Medicinal Plants Used by the Sangtam naga Tribe in Kiphire District, Nagaland, India. International Journal of Ayurvedic and Herbal Medicine.2(2 ), 267-275
Siahaan, AV and Chan, A. 2018. Formulation of Burn Gel Preparations from Ethanol Extract of Pegagan Leaves (Centella asiatica L) and Papaya Leaves (Carica papaya L). Journal of the World of Pharmacy. 2(2), 59-69
Sumoza, NS, Efrizal and Rahayu, R. 2014. Effect of Giving Gambir (Uncaria gambir R.) on Burn Healing in Male White (Mus musculus L.) Mice. Andalas University Biology Journal. 3(4), 1-11
Sutrisno, T., Huda, N., Cahaya, N., & Srikartika, M. 2016. The Effectiveness of Gel Quercetin in Burn Healing Degree II. Media Pharmaceutica Indonesia, 1(1), 1-5
Wang, N., Wang, DM, Shen, JG, Wang, ZY, Luo, HB, Mo, SL, Chen, JP, Yang, DP, Loo, TY and Chen, YL 2012. Ellagic Acid, a Phenolic Compound, Exerts Anti-angiogenesis Effects via VEGFR-2 Signaling Pathway in Breast Cancer. Breast Cancer Res Treat. 134:943–955. DOI 10.1007/s10549-012-1977-9.
Wadankar, GD, Malode, SN and Sarambekar SL 2011. Traditionally Used Medicinal Plants for Wound Healing in The Washim District, Maharashtra. IJPRIF. 3(4), 2080-2084
Wehantouw, FS, Manurung, S., Manurung., E. and Suryanto. 2011. Antihyperglycemic Activity of Mangosteen Peel Extract (Garcinia mangostana L.) in Sucrose Induced Rats. Chem. prog. 4, 89-96
Wijaya, BA, Citraningtyas, G. and Wehantouw, F. 2014. Potential of Taro Leaf Stem Ethanol Extract (Colocasia esculenta L.) as an Alternative Medicine for Rabbit Skin Wounds (Oryctolagus cuniculus). Pharmacon. 3(3), 211-21
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Published
2022-02-22
How to Cite
Harlis, W. O. ., Indrawati, & Akbar, M. D. . (2022). The Effectiveness of Taro Leaf Stalk (Colocasia esculenta L.) Ointment Extract on Burn Wound Healing in mice (Mus musculus L.). Proceeding International Conference on Religion, Science and Education, 1, 553–560. Retrieved from https://sunankalijaga.org/prosiding/index.php/icrse/article/view/836
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