Isolation and Identification of Chemical Components of Winged Bean (Psophocarpus tetragonolobus D.C) Leaf Diethyl Ether Extract

Abstract

This study aims to identify the chemical content of winged bean leaves which were first made into extracts with variations in solvent composition and will be identified using 2 Dimensional Thin Layer Chromatography (TLC) and IR Spectrovotometry. The results process of the n butanol fraction using chloroform-methanol (8:2) developer liquid, 3 (three) spots of the compound were obtained where 3 stain bands were formed on the preparative thin layer chromatography plate. The three bands were scraped and dissolved and then purified with developer liquid on the isolates, new spots were obtained from the separation of spots of fraction C, then sprayed with 10% sulfuric acid and green-yellow-green stains were obtained with an Rf value of 0.89, while the fraction A and B obtained two stains after spraying 10% Sulfuric Acid with Rf values of 0.5 (yellow) and 0.49 (yellowish red). Band I having a maximum wavelength of 309.1 indicating the presence of a conjugated double bond at -phi* and band II have a maximum wavelength of 370.2.