Tapak Liman (Elephantopus scaber L) as Imunostimulator and Its Effect on Lymphocyte Differentiation in Mice BALB/C

Abstract

Tapak liman (Elephantopus scaber, L)  is  one  of  the  plants  that  have  medicinal properties  and  diverse roles  and  efforts of  maintenance,  improvement  and  restoration  of health  and  disease  treatment.  The  purpose of  this  study  was  to  determine  the  effect of extracts of Tapak Liman (Elephantopus scaber L) as imuno stimulator to the development of lymphocytes in mice BALB / C The procedure of this study was to test extracts aquades in vivo with various treatments (control, treatment of 0.5 g / kg, 1.0 g/ kg, 2.0 g / kg) in mice BALB / C healthy for 2 weeks. After the treatment carried out analysis of the percentage and number of cells that express CD4+, CD8+  and CD4+  CD8 +  in thymus organ, using flowcytometry. Analysis of data using one-way ANOVA followed Tukey's test with SPSS. From the analysis showed that  the  extract  of  Tapak  liman  at  various  doses  showed  no  significant  effect  on  the percentage expression of CD4 + CD8 + and CD4 + CD8 + in thymus organs. While the analysis of the number of cells, extracts of Tapak  liman show its effect on the number of cells that express CD4+, CD8 +  and CD4 + CD8 + in thymus organs. Concentration of 1.0g/kg of mice showed a good effect on the increase in T helper cells (CD4 +), cytotoxic T cells (CD8 +) and Prothymosit cells (CD4 + CD8 +).