Tapak Liman (Elephantopus scaber L) as Imunostimulator and Its Effect on Lymphocyte Differentiation in Mice BALB/C
Keywords:Tapak Liman, Imunostimulator, Lymphocyte
Tapak liman (Elephantopus scaber, L) is one of the plants that have medicinal properties and diverse roles and efforts of maintenance, improvement and restoration of health and disease treatment. The purpose of this study was to determine the effect of extracts of Tapak Liman (Elephantopus scaber L) as imuno stimulator to the development of lymphocytes in mice BALB / C The procedure of this study was to test extracts aquades in vivo with various treatments (control, treatment of 0.5 g / kg, 1.0 g/ kg, 2.0 g / kg) in mice BALB / C healthy for 2 weeks. After the treatment carried out analysis of the percentage and number of cells that express CD4+, CD8+ and CD4+ CD8 + in thymus organ, using flowcytometry. Analysis of data using one-way ANOVA followed Tukey's test with SPSS. From the analysis showed that the extract of Tapak liman at various doses showed no significant effect on the percentage expression of CD4 + CD8 + and CD4 + CD8 + in thymus organs. While the analysis of the number of cells, extracts of Tapak liman show its effect on the number of cells that express CD4+, CD8 + and CD4 + CD8 + in thymus organs. Concentration of 1.0g/kg of mice showed a good effect on the increase in T helper cells (CD4 +), cytotoxic T cells (CD8 +) and Prothymosit cells (CD4 + CD8 +).