Potential Cytotoxic on Breast Cancer Cells Line and Antioxidant of Water Extract of Catharanthus roseus [L] G.Don., Dendropthoe petandra L., Curcuma mangga Val., Piper betle L.

Authors

  • Wahyu Widowati Maranatha Christian University
  • Tjandrawati Mozef Indonesian Institute of Sciences
  • Chandra Risdian Indonesian Institute of Sciences
  • Hana Ratnawati Maranatha Christian University
  • Susy Tjahjani Maranatha Christian University
  • Ferry Sandra Trisakti University
  • Lusiana Darsono Maranatha Christian University
  • Sri Utami Sugeng Maranatha Christian University

Keywords:

cytotoxic, antioxidant, Catharanthus roseus, Dendropthoe petandra, Curcuma mangga, Piper betle, water extract, breast cancer, T47D

Abstract

Breast cancer is the most common cancer among women and the second leading cause of cancer deaths in women today. The madagascar prewinkle (Catharanthus roseus [L] G.Don), mango parasite (Dendropthoe petandra L.), white saffron (Curcuma mangga Val), betel leaves (Piper betle L.) have been reported to exhibit antioxidant,  antimutation and cytotoxic that suggested the chemopreventive potential  against various cancer including breast cancer. This research was conducted to investigate  cytotoxic activity on breast cancer cell line T47D, antioxidant activity of C. roseus, D. petandra, C. mangga and P. betle water extracts. The cytotoxic potency was determined with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The antioxidant activities were determined  by using in vitro assay  of  2,2-diphenyl-1-picrylhydrazyl (DPPH)  scavenging activity. C. roseus water extract  was able to inhibit T47D cell proliferation with IC50 4%, D. petandra  with IC50 1%, C. mangga with IC50  14% and P. betle  with IC50 3%. The highest DPPH scavenging activity of  C. roseus was 71.87%, D. petandra was 75.11%, C. mangga was 38.45% and  P. betle water extract  was  83%. We suggest that D. petandra, P. betle and C. roseus water extract   have a potential cytotoxic and antioxidant activities compared with C. mangga water extract.

Downloads

Published

2011-10-31

How to Cite

Widowati, W. ., Mozef, T. ., Risdian, C. ., Ratnawati, H. ., Tjahjani, S. ., Sandra, F. ., Darsono, L. ., & Sugeng, S. U. . (2011). Potential Cytotoxic on Breast Cancer Cells Line and Antioxidant of Water Extract of Catharanthus roseus [L] G.Don., Dendropthoe petandra L., Curcuma mangga Val., Piper betle L. Proceeding ICBB (The International Conference on Bioscience and Biotechnology), 1(1), B56-B64. Retrieved from http://sunankalijaga.org/prosiding/index.php/icbb/article/view/169

Issue

Section

Articles